KMID : 1134820120410020161
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Journal of the Korean Society of Food Science and Nutrition 2012 Volume.41 No. 2 p.161 ~ p.167
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Anti-Obesity Effect of Ethyl Acetate Extracts from Agrimonia pilosa Ledeb. in 3T3-L1 Preadipocytes
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Lee Jung-A
Ahn Eun-Kyung Hong Seong-Su Oh Joa-Sub
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Abstract
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To evaluate the anti-obesity effect of Agrimonia pilosa L., this study investigated that ethyl acetate extract from A. pilosa L. (EAAP) suppresses lipid accumulation and inhibits expression of adipogenic marker genes, such as peroxisome proliferator activated receptor ¥ã (PPAR¥ã), CCAAT-enhancer-binding protein ¥á (C/EBP¥á), glucose transporter 4 (GLUT4), and adiponectin in 3T3-L1 preadipocytes. We demonstrated that EAAP inhibited adipocyte differentiation and expression of PPAR¥ã and C/EBP¥á mRNA levels in a dose-dependent manner. In addition, EAAP reduced the PPAR¥ã transcriptional activity stimulated by rosiglitazone in HEK 293T cells and decreased the expression of GLUT4 and adiponectin in 3T3-L1 cells. These results suggest that EAAP inhibits preadipocyte differentiation and adipogenesis by blocking of PPAR¥ã and C/EBP¥á gene expression in 3T3-L1 cells.
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KEYWORD
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Agrimonia pilosa Ledeb., adipogenesis, PPAR¥ã, C/EBP¥á, 3T3-L1 cells
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